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KMID : 0903519850280010036
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1985 Volume.28 No. 1 p.36 ~ p.47
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Studies on the HIS5 Gene of Yeast - HIS5 - lacZ fusion and regulation in Saccharomyces cerevisiae -
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Abstract
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HISS gene of Saccaromyces cerevisiae was cloned into pBR 322 and also into pSH 610 shuttle vector. HIS_5 gene was expressed as promoter of lactose operon (lacZ). Ahd HIS_5-lacZ fusion was intergrated into chromosome Ⅲ of yeast. HIS_5 gene in yeast growth was controlled general amino acid control mechanism.
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